An efficient protocol for perennial ryegrass mesophyll protoplast isolation and transformation, and its application on interaction study between LpNOL and LpNYC1.
Identifieur interne : 001528 ( Main/Exploration ); précédent : 001527; suivant : 001529An efficient protocol for perennial ryegrass mesophyll protoplast isolation and transformation, and its application on interaction study between LpNOL and LpNYC1.
Auteurs : Guohui Yu [République populaire de Chine] ; Qiang Cheng [République populaire de Chine] ; Zheni Xie [République populaire de Chine] ; Bin Xu [République populaire de Chine] ; Bingru Huang [États-Unis] ; Bingyu Zhao [États-Unis]Source :
- Plant methods [ 1746-4811 ] ; 2017.
Abstract
BACKGROUND
Perennial ryegrass (
RESULTS
In this report, a highly efficient protoplast isolation (5.6 × 10
CONCLUSION
This efficient protoplast isolation and transformation protocol is sufficient for studies on protein subcellular localization and protein-protein interaction, and shall be suitable for many other molecular biology applications where the mesophyll protoplast system is desirable in perennial ryegrass.
DOI: 10.1186/s13007-017-0196-0
PubMed: 28592987
PubMed Central: PMC5460552
Affiliations:
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Improvement, Nanjing Forestry University, Nanjing, 210037 People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing Forestry University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Xie, Zheni" sort="Xie, Zheni" uniqKey="Xie Z" first="Zheni" last="Xie">Zheni Xie</name><affiliation wicri:level="1"><nlm:affiliation>College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, 210095 People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agro-grassland Science, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Bin" sort="Xu, Bin" uniqKey="Xu B" first="Bin" last="Xu">Bin 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Qiang" uniqKey="Cheng Q" first="Qiang" last="Cheng">Qiang Cheng</name><affiliation wicri:level="1"><nlm:affiliation>Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing Forestry University, Nanjing, 210037 People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing Forestry University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Xie, Zheni" sort="Xie, Zheni" uniqKey="Xie Z" first="Zheni" last="Xie">Zheni Xie</name><affiliation wicri:level="1"><nlm:affiliation>College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, 210095 People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agro-grassland Science, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Xu, Bin" sort="Xu, Bin" uniqKey="Xu B" first="Bin" last="Xu">Bin Xu</name><affiliation wicri:level="1"><nlm:affiliation>College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, 210095 People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>College of Agro-grassland Science, Nanjing Agricultural University, Nanjing</wicri:regionArea><wicri:noRegion>Nanjing</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Bingru" sort="Huang, Bingru" uniqKey="Huang B" first="Bingru" last="Huang">Bingru Huang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Plant Biology and Pathology, Rutgers, the State University of New Jersey, New Brunswick, NJ 08901 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">New Jersey</region></placeName><wicri:cityArea>Department of Plant Biology and Pathology, Rutgers, the State University of New Jersey, New Brunswick</wicri:cityArea></affiliation></author><author><name sortKey="Zhao, Bingyu" sort="Zhao, Bingyu" uniqKey="Zhao B" first="Bingyu" last="Zhao">Bingyu Zhao</name><affiliation wicri:level="2"><nlm:affiliation>Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061 USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><placeName><region type="state">Virginie</region></placeName><wicri:cityArea>Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg</wicri:cityArea></affiliation></author></analytic><series><title level="j">Plant methods</title><idno type="ISSN">1746-4811</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Perennial ryegrass (</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>In this report, a highly efficient protoplast isolation (5.6 × 10</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSION</b></p><p>This efficient protoplast isolation and transformation protocol is sufficient for studies on protein subcellular localization and protein-protein interaction, and shall be suitable for many other molecular biology applications where the mesophyll protoplast system is desirable in perennial ryegrass.</p></div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">28592987</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>29</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1746-4811</ISSN><JournalIssue CitedMedium="Print"><Volume>13</Volume><PubDate><Year>2017</Year></PubDate></JournalIssue><Title>Plant methods</Title><ISOAbbreviation>Plant Methods</ISOAbbreviation></Journal><ArticleTitle>An efficient protocol for perennial ryegrass mesophyll protoplast isolation and transformation, and its application on interaction study between LpNOL and LpNYC1.</ArticleTitle><Pagination><MedlinePgn>46</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/s13007-017-0196-0</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Perennial ryegrass (<i>Lolium perenne</i> L.) is an important temperate grass used for turf and forage purposes. With the increasing accumulation of genomic and transcriptomic data of perennial ryegrass, an efficient protoplast and transient gene expression protocol is highly desirable for in vivo gene functional studies in its homologous system.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">In this report, a highly efficient protoplast isolation (5.6 × 10<sup>7</sup> protoplasts per gram of leaf material) and transient expression (plasmid transformation efficiency at 55.2%) was developed and the detailed protocol presented. Using this protocol, the subcellular locations of two ryegrass proteins were visualized in chloroplasts and nuclei, respectively, and protein-protein interaction between two chlorophyll catabolic enzymes (LpNOL and LpNYC1) was recorded in its homologous system for the first time.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">This efficient protoplast isolation and transformation protocol is sufficient for studies on protein subcellular localization and protein-protein interaction, and shall be suitable for many other molecular biology applications where the mesophyll protoplast system is desirable in perennial ryegrass.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Guohui</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, 210095 People's Republic of China.</Affiliation><Identifier Source="ISNI">0000 0000 9750 7019</Identifier><Identifier Source="GRID">grid.27871.3b</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Qiang</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing Forestry University, Nanjing, 210037 People's Republic of China.</Affiliation><Identifier Source="GRID">grid.410625.4</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xie</LastName><ForeName>Zheni</ForeName><Initials>Z</Initials><AffiliationInfo><Affiliation>College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, 210095 People's Republic of China.</Affiliation><Identifier Source="ISNI">0000 0000 9750 7019</Identifier><Identifier Source="GRID">grid.27871.3b</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Bin</ForeName><Initials>B</Initials><Identifier Source="ORCID">0000-0002-9701-7583</Identifier><AffiliationInfo><Affiliation>College of Agro-grassland Science, Nanjing Agricultural University, Nanjing, 210095 People's Republic of China.</Affiliation><Identifier Source="ISNI">0000 0000 9750 7019</Identifier><Identifier Source="GRID">grid.27871.3b</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Bingru</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Plant Biology and Pathology, Rutgers, the State University of New Jersey, New Brunswick, NJ 08901 USA.</Affiliation><Identifier Source="ISNI">0000 0004 1936 8796</Identifier><Identifier Source="GRID">grid.430387.b</Identifier></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Bingyu</ForeName><Initials>B</Initials><AffiliationInfo><Affiliation>Department of Horticulture, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061 USA.</Affiliation><Identifier Source="ISNI">0000 0001 0694 4940</Identifier><Identifier Source="GRID">grid.438526.e</Identifier></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>06</Month><Day>05</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Plant Methods</MedlineTA><NlmUniqueID>101245798</NlmUniqueID><ISSNLinking>1746-4811</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Lolium</Keyword><Keyword MajorTopicYN="N">Protoplast</Keyword><Keyword MajorTopicYN="N">Ryegrass</Keyword><Keyword MajorTopicYN="N">Transient gene expression</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>05</Month><Day>08</Day></PubMedPubDate><PubMedPubDate 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Bin" uniqKey="Xu B" first="Bin" last="Xu">Bin Xu</name></country><country name="États-Unis"><region name="New Jersey"><name sortKey="Huang, Bingru" sort="Huang, Bingru" uniqKey="Huang B" first="Bingru" last="Huang">Bingru Huang</name></region><name sortKey="Zhao, Bingyu" sort="Zhao, Bingyu" uniqKey="Zhao B" first="Bingyu" last="Zhao">Bingyu Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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